Rebecca P. Hughey, PhD

Conceptual Schematic

THE THREE MOST SIGNIFICANT QUESTIONS THAT DEFINE YOUR RESEARCH PROGRAM:
What physiologically significant features regulate the function of the epithelial sodium channel (ENaC) in kidney and airways?

How does Mucin 1 (human MUC1, mouse Muc1) enhance the HIF-1 and beta-catenin protective pathways during acute kidney injury?

Why does a frame-shift mutation in Mucin 1 cause only kidney disease?

FIVE KEYWORDS THAT DESCRIBE YOUR AREAS OF INTEREST:
Epithelial sodium channels (ENaC) • Mucin 1 (human MUC1 and rodent Muc1) • Medullary Cystic Kidney Disease type 1 (MCKD1) or MUC1 Kidney Disease (MKD) • Acute kidney injury • Apical targeting in polarized epithelial cells

TECHNIQUES, MODELS, METHODS, ANALYTIC APPROACHES, ETC:
Co-immunoprecipitations and immunoblotting from transient and stably transfected cell lines
Pulse-chase studies in polarized epithelial cells on filter inserts using cell surface biotinylation (surface biosynthetic delivery, endocytosis and recycling)
Proteolytic cleavage and activation of ENaC in cultured cells
Glycobiology: galectin and lectin pull-down assays
Palmitoylation assays: biotin-exchange biochemistry