Brett Kaufman, PhD

Conceptual Schematic

THE THREE MOST SIGNIFICANT QUESTIONS THAT DEFINE YOUR RESEARCH PROGRAM:
What protects mitochondrial genome stability?
How broadly does mitochondrial dysfunction and damage contribute to human disease?
How does mitochondrial metabolism regulate the release of mitochondrial DNA as a signaling molecule?

FIVE KEYWORDS THAT DESCRIBE YOUR AREAS OF INTEREST:
Mitochondrial oxidative phosphorylation, Mitochondrial oxidative damage, Mitochondrial DNA metabolism and damage, G-quadruplex function and regulation

TECHNIQUES, MODELS, METHODS, ANALYTIC APPROACHES, ETC:
Mouse models of mitochondrial DNA instability (CRISPR-CAS9, cellular and whole animal metabolic phenotyping)

mtDNA content and damage as a diagnostic for mitochondrial stress (high-throughput qPCR and high-precision dPCR detection)

Mitochondrial genome replication, transcription, supercoiling, and damage (2D gel analysis)

Nuclear and mitochondrial gene expression changes in response to mitochondrial stress (qRT-PCR methods)

Mitochondrial post translational modification (2D-IEF methods)