Patrick J. Pagano, PhD

Conceptual Schematic

THE THREE MOST SIGNIFICANT QUESTIONS THAT DEFINE YOUR RESEARCH PROGRAM:
What are the signaling mechanisms regulating NADPH oxidase (Nox) action and vascular cell phenotype?

How are Noxs involved in the pathogenesis of cardiovascular & pulmonary diseases?

Can we design new isoform-specific inhibitors to dissect isoform-unique functions and be developed into drug therapies?

FIVE KEYWORDS THAT DESCRIBE YOUR AREAS OF INTEREST:
NADPH Oxidase family of proteins and its regulators, Reactive oxygen/nitrogen species (ROS/RNS), Signal transduction, Cardiovascular disease, Druggable peptidic & small molecules Nox inhibitors

TECHNIQUES, MODELS, METHODS, ANALYTIC APPROACHES, ETC:
Rigorous high- and low-throughput ROS-detection assays
Biochemical & imaging analysis of protein : protein interaction (e.g., FRET, FRAP, ICS, IVIS)
In vivo models of oxidative stress-driven cardiovascular diseases; generation of new endothelial- and smooth muscle-specific knockout mice
Vessel myography of vascular tone development • Laser Doppler in vivo vascular flow analysis