Sruti Shiva, PhD

Conceptual Schematic

THE THREE MOST SIGNIFICANT QUESTIONS THAT DEFINE YOUR RESEARCH PROGRAM:
Can platelet bioenergetics/mitochondrial function be utilized as a biomarker of disease progression and therapeutic efficacy?

How does red blood cell hemolysis cause platelet mitochondrial dysfunction and pathological signaling in hemolytic diseases?

How does the endogenous signaling molecule nitrite interact with the oxygen carrier myoglobin to regulate mitochondrial function and mediate cardioprotection?

FIVE KEYWORDS THAT DESCRIBE YOUR AREAS OF INTEREST:
Mitochondrial function/bioenergetics, Nitrite/nitric oxide, Reactive oxygen species, Hypoxia, Myoglobin

TECHNIQUES, MODELS, METHODS, ANALYTIC APPROACHES, ETC:
Seahorse extracellular flux analysis
Kinetic assays for mitochondrial enzymatic function
Human platelet bioenergetics
Measurement of mitochondrial function in models ranging from isolated mitochondria to primary & cultured cells to in vivo animal models and human circulating cells
Nitric oxide and Reactive Oxygen Species measurement